氧化石墨烯增强的骨仿生静电纺左旋聚乳酸纳米纤维支架的制备及表征

材料科学与工程学报, 2016, 34(4): 535-555. doi: 10.14136/j.cnki.issn 1673-2812.2016.04.005

氧化石墨烯增强的骨仿生静电纺左旋聚乳酸纳米纤维支架的制备及表征

邵伟力 ^1, , 桑锋 ^2, , 何书杰 ^3, , 钱洁玉 ^4, , 李强 ^5, , 王倩 ^6,

1.天津工业大学复合材料研究所先进纺织复合材料教育部重点实验室,天津,300387

2.河南中医学院第一附属医院,河南郑州,450000

3.河南中医学院第一附属医院,河南郑州,450000

4.河南中医学院第一附属医院,河南郑州,450000

5.河南中医学院第一附属医院,河南郑州,450000

6.河南省病毒性疾病中医药防治重点实验室,河南郑州,450000

基金项目: 河南省教育厅科学技术研究重点资助项目(14A540003,14A540006) 郑州市普通科技攻关资助项目(141PPTGG400) NSFC-河南人才培养联合基金资助项目(U1204510) 国家自然科学基金资助项目(51203196,U1204510)

关键词：聚左旋乳酸 , 氧化石墨烯 , 力学性能 , 生物学性能

Preparation and Characterization of Bonelike Biomimetic Electrospun Poly L-lactic Acid Nanofibers Enhanced by Graphene Oxide

Keywords： Poly l-lactic (PLLA) , Grapheme oxide , Mechanical properties , Biological properties

利用静电纺丝技术制备了左旋聚乳酸/氧化石墨烯(PLA/GO)复合纳米纤维毡.通过扫描电子显微镜(SEM)、透射电子显微镜(TEM)、孔隙率测试、傅里叶红外光谱分析(FTIR)以及拉伸测试分别对PLLA/GO纳米纤维的形貌结构、孔隙率及力学性能进行了研究.将小鼠骨髓间充质干细胞(MSCs)种植在TSF/PLLA纳米纤维上,通过荧光显微镜分析和碱性磷酸酶(ALP)测试、SEM观察细胞在材料表面的生长以及矿物沉积情况评价复合纳米纤维的生物学性能.结果表明,与纯的PLLA静电纺纳米纤维支架相比,PLLA/GO复合纳米纤维支架的纤维直径显著减小,孔隙率增大,力学性能明显得到改善,拉伸强度和杨氏模量均高于纯PLLA纳米纤维支架将近3倍,而且能够更好地促进MSCs的粘附、增殖和分化.

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